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pe vio770 mouse anti human cd25  (Miltenyi Biotec)


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    Miltenyi Biotec pe vio770 mouse anti human cd25
    SIV-specific T cell responses and correlation with viral rebound AUC (A) SIVgag-specific CD8 + T cell responses were quantified by AIM assay, using Boolean gating to show the frequencies of CD69 + <t>CD25</t> + , CD69 + OX40 + , or CD25 + OX40 + cells following stimulation with an SIVgag peptide pool after background subtraction. The pre-ATI (and pre-RhmAb for that group) time point was compared to the rebound peak post-ATI. Nonparametric Wilcoxon matched-pairs signed rank test was used for statistics. N = 4 and N = 5 for controls at each time point based on sample availability. (B) Graph shows the frequency of SIVgag-specific CD8 + T cells at peak rebound in the RhmAb group and rebound viral load AUC. Statistical analysis was performed by calculating the nonparametric Spearman’s rank correlation coefficient (r) that was compared to zero.
    Pe Vio770 Mouse Anti Human Cd25, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pe+anti+mouse+cd25/CD25+Antibody%2C+anti-human%2C+REAfinity/pmc13006440-12-0-5
    Average 93 stars, based on 76 article reviews
    pe vio770 mouse anti human cd25 - by Bioz Stars, 2026-10
    93/100 stars

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    1) Product Images from "Delayed viral rebound post-ART interruption in infant macaques given SIV-specific neutralizing antibodies"

    Article Title: Delayed viral rebound post-ART interruption in infant macaques given SIV-specific neutralizing antibodies

    Journal: Cell Reports Medicine

    doi: 10.1016/j.xcrm.2026.102636

    SIV-specific T cell responses and correlation with viral rebound AUC (A) SIVgag-specific CD8 + T cell responses were quantified by AIM assay, using Boolean gating to show the frequencies of CD69 + CD25 + , CD69 + OX40 + , or CD25 + OX40 + cells following stimulation with an SIVgag peptide pool after background subtraction. The pre-ATI (and pre-RhmAb for that group) time point was compared to the rebound peak post-ATI. Nonparametric Wilcoxon matched-pairs signed rank test was used for statistics. N = 4 and N = 5 for controls at each time point based on sample availability. (B) Graph shows the frequency of SIVgag-specific CD8 + T cells at peak rebound in the RhmAb group and rebound viral load AUC. Statistical analysis was performed by calculating the nonparametric Spearman’s rank correlation coefficient (r) that was compared to zero.
    Figure Legend Snippet: SIV-specific T cell responses and correlation with viral rebound AUC (A) SIVgag-specific CD8 + T cell responses were quantified by AIM assay, using Boolean gating to show the frequencies of CD69 + CD25 + , CD69 + OX40 + , or CD25 + OX40 + cells following stimulation with an SIVgag peptide pool after background subtraction. The pre-ATI (and pre-RhmAb for that group) time point was compared to the rebound peak post-ATI. Nonparametric Wilcoxon matched-pairs signed rank test was used for statistics. N = 4 and N = 5 for controls at each time point based on sample availability. (B) Graph shows the frequency of SIVgag-specific CD8 + T cells at peak rebound in the RhmAb group and rebound viral load AUC. Statistical analysis was performed by calculating the nonparametric Spearman’s rank correlation coefficient (r) that was compared to zero.

    Techniques Used:

    Related Articles

    other:

    Article Title: Valrubicin-loaded immunoliposomes targeting antigens on immunosuppressive cells to circumvent resistance to cancer immunotherapy
    Article Snippet: PE anti-Mouse CD25 (Clone REA568) , Miltenyi Biotec , Cat# 130-120-696; RRID: AB_2752168.

    Immunohistochemistry:

    Article Title: Fucoxanthin Ameliorates Atopic Dermatitis Symptoms by Regulating Keratinocytes and Regulatory Innate Lymphoid Cells
    Article Snippet: .. Sections were subjected to immunohistochemistry using antibodies of Alexa Fluor 488 ® -anti-mouse Il-10 (#505013), Brilliant Violet 421TM-anti-mouse TGF-β1 (#141407), Pacific BlueTM-anti-mouse IL-2 (#503820), Alexa Fluor ® 488-anti-GATA3 (#653808), PE-anti-mouse Ly-6A/E (Sca-1) (#108108), PE-anti-mouse IL-33Rα (ST2) (#146608) and PE-anti-mouse/human IL-5 (#504304) (BioLegend, San Diego, CA, USA), FITC-anti-mouse/human CD45R (#11-0452-82) (Thermo Fisher Scientific, Waltham, MA, USA), PE-anti-mouse CD25 (#130-120-766) (Miltenyi Biotech, Bergisch Gladbach, Germany). ..



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    SIV-specific T cell responses and correlation with viral rebound AUC (A) SIVgag-specific CD8 + T cell responses were quantified by AIM assay, using Boolean gating to show the frequencies of CD69 + <t>CD25</t> + , CD69 + OX40 + , or CD25 + OX40 + cells following stimulation with an SIVgag peptide pool after background subtraction. The pre-ATI (and pre-RhmAb for that group) time point was compared to the rebound peak post-ATI. Nonparametric Wilcoxon matched-pairs signed rank test was used for statistics. N = 4 and N = 5 for controls at each time point based on sample availability. (B) Graph shows the frequency of SIVgag-specific CD8 + T cells at peak rebound in the RhmAb group and rebound viral load AUC. Statistical analysis was performed by calculating the nonparametric Spearman’s rank correlation coefficient (r) that was compared to zero.
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    Image Search Results


    SIV-specific T cell responses and correlation with viral rebound AUC (A) SIVgag-specific CD8 + T cell responses were quantified by AIM assay, using Boolean gating to show the frequencies of CD69 + CD25 + , CD69 + OX40 + , or CD25 + OX40 + cells following stimulation with an SIVgag peptide pool after background subtraction. The pre-ATI (and pre-RhmAb for that group) time point was compared to the rebound peak post-ATI. Nonparametric Wilcoxon matched-pairs signed rank test was used for statistics. N = 4 and N = 5 for controls at each time point based on sample availability. (B) Graph shows the frequency of SIVgag-specific CD8 + T cells at peak rebound in the RhmAb group and rebound viral load AUC. Statistical analysis was performed by calculating the nonparametric Spearman’s rank correlation coefficient (r) that was compared to zero.

    Journal: Cell Reports Medicine

    Article Title: Delayed viral rebound post-ART interruption in infant macaques given SIV-specific neutralizing antibodies

    doi: 10.1016/j.xcrm.2026.102636

    Figure Lengend Snippet: SIV-specific T cell responses and correlation with viral rebound AUC (A) SIVgag-specific CD8 + T cell responses were quantified by AIM assay, using Boolean gating to show the frequencies of CD69 + CD25 + , CD69 + OX40 + , or CD25 + OX40 + cells following stimulation with an SIVgag peptide pool after background subtraction. The pre-ATI (and pre-RhmAb for that group) time point was compared to the rebound peak post-ATI. Nonparametric Wilcoxon matched-pairs signed rank test was used for statistics. N = 4 and N = 5 for controls at each time point based on sample availability. (B) Graph shows the frequency of SIVgag-specific CD8 + T cells at peak rebound in the RhmAb group and rebound viral load AUC. Statistical analysis was performed by calculating the nonparametric Spearman’s rank correlation coefficient (r) that was compared to zero.

    Article Snippet: PE-Vio770 Mouse Anti-Human CD25 , Miltenyi Biotec , 130-113-287; RRID:AB_2784092.

    Techniques:

    DNase I treatment decreased the number of immune inflammatory cells in the kidneys and spleens of mice in the MRL/lpr model. (A, B) The TIMER algorithm was used to assess the proportion of immune cell infiltration in the kidneys of control (Ctr), MRL/lpr (Lpr), and DNase I-treated MRL/lpr (DNase) mice at 21 weeks of age. The percentages of CD3+CD4+ T cell subsets (C, G) , CD3+CD8+ T cell subsets (D, H) , and CD3-CD19+B cell subsets (E, I) in the spleen were quantified using flow cytometry across the normal control, MRL/lpr, and DNase I treatment MRL/lpr group. (F, J) Flow cytometric and quantitative analysis of CD4+CD25+Foxp3+ regulatory T cell (Treg) percentages in the spleens of mice from each experimental group were also conducted. Data were expressed as means ± SD for groups of three mice. * P < 0.05, ** P < 0.01, and *** P < 0.001 vs. normal control ( t test). # P < 0.05 vs. MRL/lpr mice (Bonferroni correction; two comparisons were made).

    Journal: Frontiers in Immunology

    Article Title: DNase I alleviates renal inflammatory injury in MRL/lpr mice by inhibiting NETs formation

    doi: 10.3389/fimmu.2025.1656069

    Figure Lengend Snippet: DNase I treatment decreased the number of immune inflammatory cells in the kidneys and spleens of mice in the MRL/lpr model. (A, B) The TIMER algorithm was used to assess the proportion of immune cell infiltration in the kidneys of control (Ctr), MRL/lpr (Lpr), and DNase I-treated MRL/lpr (DNase) mice at 21 weeks of age. The percentages of CD3+CD4+ T cell subsets (C, G) , CD3+CD8+ T cell subsets (D, H) , and CD3-CD19+B cell subsets (E, I) in the spleen were quantified using flow cytometry across the normal control, MRL/lpr, and DNase I treatment MRL/lpr group. (F, J) Flow cytometric and quantitative analysis of CD4+CD25+Foxp3+ regulatory T cell (Treg) percentages in the spleens of mice from each experimental group were also conducted. Data were expressed as means ± SD for groups of three mice. * P < 0.05, ** P < 0.01, and *** P < 0.001 vs. normal control ( t test). # P < 0.05 vs. MRL/lpr mice (Bonferroni correction; two comparisons were made).

    Article Snippet: The following antibodies were utilized for staining: anti-CD3 antibody (E-AB-F1013Q), anti-CD4 antibody (E-AB-F1097S), anti-CD8 antibody (E-AB-F1104J), anti-CD19 antibody (E-AB-F0986E), anti-CD25 antibody (E-AB-F1102D), and anti-Foxp3 antibody (E-AB-F1238E), all sourced from Elabscience (China).

    Techniques: Control, Flow Cytometry